Review





Similar Products

plasmidsaurus bioinformatic rna seq pipeline  (Plasmidsaurus)
99
Plasmidsaurus plasmidsaurus bioinformatic rna seq pipeline
A) Schematic of human mitochondrial DNA expression. mt-mRNAs are colored by OXPHOS complex. mt-rRNAs are shown in orange and mt-tRNAs in dark grey. TSS, transcription start site. B) Representative Western blot analysis of OXPHOS subunits (left) and quantification of protein levels (right) from three replicate measurements of whole cell lysate from NT, FASTKD4 KO, and FASTKD4 KO rescue cells. Error bars represent standard deviation. Brown-Forsythe and Welch ANOVA test followed by Dunnett’s T3 multiple comparisons test, and significant p-values are indicated; *p-value < 0.05. kDa, kilodalton; EV, empty vector. C) Volcano plot depicting log2 fold change (FASTKD4 KO/NT control; x-axis) and -log10 adjusted p-value (y-axis) from <t>RNA-seq.</t> mt-mRNAs are highlighted. D) NT-normalized transcript abundance (relative to ACTB) for a subset of mt-mRNAs as quantified by RT-qPCR for three replicate measurements from NT, FASTKD4 KO, and FASTKD4-FLAG rescue cells. Error bars represent standard deviation. Brown-Forsythe and Welch ANOVA test followed by Dunnett’s T3 multiple comparisons test, and significant p-values are indicated; *p-value < 0.05 and **p-value < 0.01.
Plasmidsaurus Bioinformatic Rna Seq Pipeline, supplied by Plasmidsaurus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bioinformatics+pipeline/RNA-Seq/bio_rxiv__64898__2026__07__30__741833-84-11-11
Average 99 stars, based on 1 article reviews
plasmidsaurus bioinformatic rna seq pipeline - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
sophia ddm bioinformatics pipeline  (Sophia Genetics)
97
Sophia Genetics sophia ddm bioinformatics pipeline
A) Schematic of human mitochondrial DNA expression. mt-mRNAs are colored by OXPHOS complex. mt-rRNAs are shown in orange and mt-tRNAs in dark grey. TSS, transcription start site. B) Representative Western blot analysis of OXPHOS subunits (left) and quantification of protein levels (right) from three replicate measurements of whole cell lysate from NT, FASTKD4 KO, and FASTKD4 KO rescue cells. Error bars represent standard deviation. Brown-Forsythe and Welch ANOVA test followed by Dunnett’s T3 multiple comparisons test, and significant p-values are indicated; *p-value < 0.05. kDa, kilodalton; EV, empty vector. C) Volcano plot depicting log2 fold change (FASTKD4 KO/NT control; x-axis) and -log10 adjusted p-value (y-axis) from <t>RNA-seq.</t> mt-mRNAs are highlighted. D) NT-normalized transcript abundance (relative to ACTB) for a subset of mt-mRNAs as quantified by RT-qPCR for three replicate measurements from NT, FASTKD4 KO, and FASTKD4-FLAG rescue cells. Error bars represent standard deviation. Brown-Forsythe and Welch ANOVA test followed by Dunnett’s T3 multiple comparisons test, and significant p-values are indicated; *p-value < 0.05 and **p-value < 0.01.
Sophia Ddm Bioinformatics Pipeline, supplied by Sophia Genetics, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bioinformatics+pipeline/SOPHiA+DDM/pm42024101-49-1-1
Average 97 stars, based on 1 article reviews
sophia ddm bioinformatics pipeline - by Bioz Stars, 2026-10
97/100 stars
  Buy from Supplier
bioinformatics pipelines  (Azenta)
86
Azenta bioinformatics pipelines
A) Schematic of human mitochondrial DNA expression. mt-mRNAs are colored by OXPHOS complex. mt-rRNAs are shown in orange and mt-tRNAs in dark grey. TSS, transcription start site. B) Representative Western blot analysis of OXPHOS subunits (left) and quantification of protein levels (right) from three replicate measurements of whole cell lysate from NT, FASTKD4 KO, and FASTKD4 KO rescue cells. Error bars represent standard deviation. Brown-Forsythe and Welch ANOVA test followed by Dunnett’s T3 multiple comparisons test, and significant p-values are indicated; *p-value < 0.05. kDa, kilodalton; EV, empty vector. C) Volcano plot depicting log2 fold change (FASTKD4 KO/NT control; x-axis) and -log10 adjusted p-value (y-axis) from <t>RNA-seq.</t> mt-mRNAs are highlighted. D) NT-normalized transcript abundance (relative to ACTB) for a subset of mt-mRNAs as quantified by RT-qPCR for three replicate measurements from NT, FASTKD4 KO, and FASTKD4-FLAG rescue cells. Error bars represent standard deviation. Brown-Forsythe and Welch ANOVA test followed by Dunnett’s T3 multiple comparisons test, and significant p-values are indicated; *p-value < 0.05 and **p-value < 0.01.
Bioinformatics Pipelines, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bioinformatics+pipeline/bioinformatics+pipeline+standard/10__1158_slash_2767___9764__crc___25___0338-148-21-17
Average 86 stars, based on 1 article reviews
bioinformatics pipelines - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier
proprietary bioinformatics pipeline  (Invitae Inc)
86
Invitae Inc proprietary bioinformatics pipeline
A) Schematic of human mitochondrial DNA expression. mt-mRNAs are colored by OXPHOS complex. mt-rRNAs are shown in orange and mt-tRNAs in dark grey. TSS, transcription start site. B) Representative Western blot analysis of OXPHOS subunits (left) and quantification of protein levels (right) from three replicate measurements of whole cell lysate from NT, FASTKD4 KO, and FASTKD4 KO rescue cells. Error bars represent standard deviation. Brown-Forsythe and Welch ANOVA test followed by Dunnett’s T3 multiple comparisons test, and significant p-values are indicated; *p-value < 0.05. kDa, kilodalton; EV, empty vector. C) Volcano plot depicting log2 fold change (FASTKD4 KO/NT control; x-axis) and -log10 adjusted p-value (y-axis) from <t>RNA-seq.</t> mt-mRNAs are highlighted. D) NT-normalized transcript abundance (relative to ACTB) for a subset of mt-mRNAs as quantified by RT-qPCR for three replicate measurements from NT, FASTKD4 KO, and FASTKD4-FLAG rescue cells. Error bars represent standard deviation. Brown-Forsythe and Welch ANOVA test followed by Dunnett’s T3 multiple comparisons test, and significant p-values are indicated; *p-value < 0.05 and **p-value < 0.01.
Proprietary Bioinformatics Pipeline, supplied by Invitae Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bioinformatics+pipeline/bioinformatics+pipeline+proprietary/pm42074219-188-12-19
Average 86 stars, based on 1 article reviews
proprietary bioinformatics pipeline - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier
standard bioinformatics pipeline  (Novogene)
86
Novogene standard bioinformatics pipeline
A) Schematic of human mitochondrial DNA expression. mt-mRNAs are colored by OXPHOS complex. mt-rRNAs are shown in orange and mt-tRNAs in dark grey. TSS, transcription start site. B) Representative Western blot analysis of OXPHOS subunits (left) and quantification of protein levels (right) from three replicate measurements of whole cell lysate from NT, FASTKD4 KO, and FASTKD4 KO rescue cells. Error bars represent standard deviation. Brown-Forsythe and Welch ANOVA test followed by Dunnett’s T3 multiple comparisons test, and significant p-values are indicated; *p-value < 0.05. kDa, kilodalton; EV, empty vector. C) Volcano plot depicting log2 fold change (FASTKD4 KO/NT control; x-axis) and -log10 adjusted p-value (y-axis) from <t>RNA-seq.</t> mt-mRNAs are highlighted. D) NT-normalized transcript abundance (relative to ACTB) for a subset of mt-mRNAs as quantified by RT-qPCR for three replicate measurements from NT, FASTKD4 KO, and FASTKD4-FLAG rescue cells. Error bars represent standard deviation. Brown-Forsythe and Welch ANOVA test followed by Dunnett’s T3 multiple comparisons test, and significant p-values are indicated; *p-value < 0.05 and **p-value < 0.01.
Standard Bioinformatics Pipeline, supplied by Novogene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bioinformatics+pipeline/bioinformatics+pipeline+standard/pm42083037-324-16-10
Average 86 stars, based on 1 article reviews
standard bioinformatics pipeline - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier
house bioinformatics pipeline  (Novogene)
86
Novogene house bioinformatics pipeline
A) Schematic of human mitochondrial DNA expression. mt-mRNAs are colored by OXPHOS complex. mt-rRNAs are shown in orange and mt-tRNAs in dark grey. TSS, transcription start site. B) Representative Western blot analysis of OXPHOS subunits (left) and quantification of protein levels (right) from three replicate measurements of whole cell lysate from NT, FASTKD4 KO, and FASTKD4 KO rescue cells. Error bars represent standard deviation. Brown-Forsythe and Welch ANOVA test followed by Dunnett’s T3 multiple comparisons test, and significant p-values are indicated; *p-value < 0.05. kDa, kilodalton; EV, empty vector. C) Volcano plot depicting log2 fold change (FASTKD4 KO/NT control; x-axis) and -log10 adjusted p-value (y-axis) from <t>RNA-seq.</t> mt-mRNAs are highlighted. D) NT-normalized transcript abundance (relative to ACTB) for a subset of mt-mRNAs as quantified by RT-qPCR for three replicate measurements from NT, FASTKD4 KO, and FASTKD4-FLAG rescue cells. Error bars represent standard deviation. Brown-Forsythe and Welch ANOVA test followed by Dunnett’s T3 multiple comparisons test, and significant p-values are indicated; *p-value < 0.05 and **p-value < 0.01.
House Bioinformatics Pipeline, supplied by Novogene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bioinformatics+pipeline/house+in+perl+scripts/bio_rxiv__64898__2026__04__23__720098-60-7-6
Average 86 stars, based on 1 article reviews
house bioinformatics pipeline - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier
bmkgene bioinformatics pipeline  (Beijing Biomarker Technologies Co Ltd)
86
Beijing Biomarker Technologies Co Ltd bmkgene bioinformatics pipeline
A) Schematic of human mitochondrial DNA expression. mt-mRNAs are colored by OXPHOS complex. mt-rRNAs are shown in orange and mt-tRNAs in dark grey. TSS, transcription start site. B) Representative Western blot analysis of OXPHOS subunits (left) and quantification of protein levels (right) from three replicate measurements of whole cell lysate from NT, FASTKD4 KO, and FASTKD4 KO rescue cells. Error bars represent standard deviation. Brown-Forsythe and Welch ANOVA test followed by Dunnett’s T3 multiple comparisons test, and significant p-values are indicated; *p-value < 0.05. kDa, kilodalton; EV, empty vector. C) Volcano plot depicting log2 fold change (FASTKD4 KO/NT control; x-axis) and -log10 adjusted p-value (y-axis) from <t>RNA-seq.</t> mt-mRNAs are highlighted. D) NT-normalized transcript abundance (relative to ACTB) for a subset of mt-mRNAs as quantified by RT-qPCR for three replicate measurements from NT, FASTKD4 KO, and FASTKD4-FLAG rescue cells. Error bars represent standard deviation. Brown-Forsythe and Welch ANOVA test followed by Dunnett’s T3 multiple comparisons test, and significant p-values are indicated; *p-value < 0.05 and **p-value < 0.01.
Bmkgene Bioinformatics Pipeline, supplied by Beijing Biomarker Technologies Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bioinformatics+pipeline/bioinformatics+bmkgene+pipeline/pmc13115689-196-6-6
Average 86 stars, based on 1 article reviews
bmkgene bioinformatics pipeline - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier
standardized bioinformatics pipeline  (Macrogen)
86
Macrogen standardized bioinformatics pipeline
A) Schematic of human mitochondrial DNA expression. mt-mRNAs are colored by OXPHOS complex. mt-rRNAs are shown in orange and mt-tRNAs in dark grey. TSS, transcription start site. B) Representative Western blot analysis of OXPHOS subunits (left) and quantification of protein levels (right) from three replicate measurements of whole cell lysate from NT, FASTKD4 KO, and FASTKD4 KO rescue cells. Error bars represent standard deviation. Brown-Forsythe and Welch ANOVA test followed by Dunnett’s T3 multiple comparisons test, and significant p-values are indicated; *p-value < 0.05. kDa, kilodalton; EV, empty vector. C) Volcano plot depicting log2 fold change (FASTKD4 KO/NT control; x-axis) and -log10 adjusted p-value (y-axis) from <t>RNA-seq.</t> mt-mRNAs are highlighted. D) NT-normalized transcript abundance (relative to ACTB) for a subset of mt-mRNAs as quantified by RT-qPCR for three replicate measurements from NT, FASTKD4 KO, and FASTKD4-FLAG rescue cells. Error bars represent standard deviation. Brown-Forsythe and Welch ANOVA test followed by Dunnett’s T3 multiple comparisons test, and significant p-values are indicated; *p-value < 0.05 and **p-value < 0.01.
Standardized Bioinformatics Pipeline, supplied by Macrogen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bioinformatics+pipeline/bioinformatics+pipeline+standardized/pm41897732-82-6-15
Average 86 stars, based on 1 article reviews
standardized bioinformatics pipeline - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier

Image Search Results


A) Schematic of human mitochondrial DNA expression. mt-mRNAs are colored by OXPHOS complex. mt-rRNAs are shown in orange and mt-tRNAs in dark grey. TSS, transcription start site. B) Representative Western blot analysis of OXPHOS subunits (left) and quantification of protein levels (right) from three replicate measurements of whole cell lysate from NT, FASTKD4 KO, and FASTKD4 KO rescue cells. Error bars represent standard deviation. Brown-Forsythe and Welch ANOVA test followed by Dunnett’s T3 multiple comparisons test, and significant p-values are indicated; *p-value < 0.05. kDa, kilodalton; EV, empty vector. C) Volcano plot depicting log2 fold change (FASTKD4 KO/NT control; x-axis) and -log10 adjusted p-value (y-axis) from RNA-seq. mt-mRNAs are highlighted. D) NT-normalized transcript abundance (relative to ACTB) for a subset of mt-mRNAs as quantified by RT-qPCR for three replicate measurements from NT, FASTKD4 KO, and FASTKD4-FLAG rescue cells. Error bars represent standard deviation. Brown-Forsythe and Welch ANOVA test followed by Dunnett’s T3 multiple comparisons test, and significant p-values are indicated; *p-value < 0.05 and **p-value < 0.01.

Journal: bioRxiv

Article Title: Mitochondrial RNA processing promotes translation by resolving structured precursor RNAs

doi: 10.64898/2026.07.30.741833

Figure Lengend Snippet: A) Schematic of human mitochondrial DNA expression. mt-mRNAs are colored by OXPHOS complex. mt-rRNAs are shown in orange and mt-tRNAs in dark grey. TSS, transcription start site. B) Representative Western blot analysis of OXPHOS subunits (left) and quantification of protein levels (right) from three replicate measurements of whole cell lysate from NT, FASTKD4 KO, and FASTKD4 KO rescue cells. Error bars represent standard deviation. Brown-Forsythe and Welch ANOVA test followed by Dunnett’s T3 multiple comparisons test, and significant p-values are indicated; *p-value < 0.05. kDa, kilodalton; EV, empty vector. C) Volcano plot depicting log2 fold change (FASTKD4 KO/NT control; x-axis) and -log10 adjusted p-value (y-axis) from RNA-seq. mt-mRNAs are highlighted. D) NT-normalized transcript abundance (relative to ACTB) for a subset of mt-mRNAs as quantified by RT-qPCR for three replicate measurements from NT, FASTKD4 KO, and FASTKD4-FLAG rescue cells. Error bars represent standard deviation. Brown-Forsythe and Welch ANOVA test followed by Dunnett’s T3 multiple comparisons test, and significant p-values are indicated; *p-value < 0.05 and **p-value < 0.01.

Article Snippet: Libraries were sequenced on an Illumina platform and processed using the Plasmidsaurus bioinformatic RNA-seq pipeline.

Techniques: Expressing, Western Blot, Standard Deviation, Plasmid Preparation, Control, RNA Sequencing, Quantitative RT-PCR

A) Western blot analysis confirming successful FASTKD4 KO in HEK293T cells for three independently generated clonal KO lines. NT, non-targeting control. B) CellTiter-Glo assay to quantify relative ATP production by NT and FASTKD4 KO cells when cultured in glucose media compared to media containing 2-DG or oligomycin. Error bars represent standard deviation. 2-DG, deoxyglucose. Two-way ANOVA followed by Sidak’s multiple comparison test, and significant p-values are indicated; ****p-value < 0.0001. C) Percentage live cells for NT and FASTKD4 KO cells cultured in either glucose media or media containing 10 mM 2-DG for 4 days, as determined by AnnexinV/PI staining and flow cytometry analysis. Two-way ANOVA followed by Sidak’s multiple comparison test; ns, not statistically significant. D) Representative Western blot analysis of OXPHOS subunits (left) and quantification of protein levels (right) from three replicate measurements of whole cell lysate from NT and FASTKD4 KO cells. Error bars represent standard deviation. Unpaired t-test with Welch’s correction, and significant p-values are indicated; *p-value < 0.05. E) Representative Western blot of OXPHOS protein stability (left) and quantification of protein levels (right) from two replicate measurements of whole cell lysate from NT and FASTKD4 KO cells after 0, 2, or 4 days of treatment with chloramphenicol (CAP). Error bars show standard deviation. Two-way ANOVA followed by Sidak’s multiple comparison test. Interaction not statistically significant unless otherwise indicated. F) Representative Western blot analysis of OXPHOS subunits (left) and quantification of protein levels (right) from three replicate measurements of whole cell lysate from NT and pooled FASTKD4 KO in HeLa. Error bars represent standard deviation. Statistical testing is the same as in D . G) Volcano plot depicting log2 fold change (FASTKD4-FLAG rescue/FASTKD4 KO; x-axis) and -log10 adjusted p-value (y-axis) from RNA-seq. mt-mRNAs are highlighted. H) NT-normalized transcript abundance (relative to ACTB) for a subset of mt-mRNAs as quantified by RT-qPCR for three replicate measurements from NT and pooled FASTKD4 KO HeLa cells. Error bars represent standard deviation. Statistical testing is the same as in D ; *p-value < 0.05, **p-value < 0.01.

Journal: bioRxiv

Article Title: Mitochondrial RNA processing promotes translation by resolving structured precursor RNAs

doi: 10.64898/2026.07.30.741833

Figure Lengend Snippet: A) Western blot analysis confirming successful FASTKD4 KO in HEK293T cells for three independently generated clonal KO lines. NT, non-targeting control. B) CellTiter-Glo assay to quantify relative ATP production by NT and FASTKD4 KO cells when cultured in glucose media compared to media containing 2-DG or oligomycin. Error bars represent standard deviation. 2-DG, deoxyglucose. Two-way ANOVA followed by Sidak’s multiple comparison test, and significant p-values are indicated; ****p-value < 0.0001. C) Percentage live cells for NT and FASTKD4 KO cells cultured in either glucose media or media containing 10 mM 2-DG for 4 days, as determined by AnnexinV/PI staining and flow cytometry analysis. Two-way ANOVA followed by Sidak’s multiple comparison test; ns, not statistically significant. D) Representative Western blot analysis of OXPHOS subunits (left) and quantification of protein levels (right) from three replicate measurements of whole cell lysate from NT and FASTKD4 KO cells. Error bars represent standard deviation. Unpaired t-test with Welch’s correction, and significant p-values are indicated; *p-value < 0.05. E) Representative Western blot of OXPHOS protein stability (left) and quantification of protein levels (right) from two replicate measurements of whole cell lysate from NT and FASTKD4 KO cells after 0, 2, or 4 days of treatment with chloramphenicol (CAP). Error bars show standard deviation. Two-way ANOVA followed by Sidak’s multiple comparison test. Interaction not statistically significant unless otherwise indicated. F) Representative Western blot analysis of OXPHOS subunits (left) and quantification of protein levels (right) from three replicate measurements of whole cell lysate from NT and pooled FASTKD4 KO in HeLa. Error bars represent standard deviation. Statistical testing is the same as in D . G) Volcano plot depicting log2 fold change (FASTKD4-FLAG rescue/FASTKD4 KO; x-axis) and -log10 adjusted p-value (y-axis) from RNA-seq. mt-mRNAs are highlighted. H) NT-normalized transcript abundance (relative to ACTB) for a subset of mt-mRNAs as quantified by RT-qPCR for three replicate measurements from NT and pooled FASTKD4 KO HeLa cells. Error bars represent standard deviation. Statistical testing is the same as in D ; *p-value < 0.05, **p-value < 0.01.

Article Snippet: Libraries were sequenced on an Illumina platform and processed using the Plasmidsaurus bioinformatic RNA-seq pipeline.

Techniques: Western Blot, Generated, Control, Glo Assay, Cell Culture, Standard Deviation, Comparison, Staining, Flow Cytometry, RNA Sequencing, Quantitative RT-PCR